論文出處：Asian-Australas J Anim Sci. 2020 Jul;33(7):1113-1125. doi: 10.5713/ajas.19.0392.
論文名稱：1. Effects of dietary Antrodia cinnamomea fermented product supplementation on antioxidation, anti-inflammation, and lipid metabolism in broiler chickens.
作 者：Lee MT, Lin WC, Lin LJ, Wang SY, Chang SC, Lee TT.
出 版 年：2020
This study was investigated the effects of dietary supplementation of Antrodia cinnamomea fermented product on modulation of antioxidation, anti-inflammation, and lipid metabolism in broilers.
Functional compounds and in vitro antioxidant capacity were detected in wheat bran (WB) solid-state fermented by Antrodia cinnamomea for 16 days (FAC). In animal experiment, 400 d-old broiler chickens were allotted into 5 groups fed control diet, and control diet replaced with 5% WB, 10% WB, 5% FAC, and 10% FAC respectively. Growth performance, intestinal microflora, serum antioxidant enzymes and fatty acid profiles in pectoral superficial muscle were measured.
Pretreatment with hot water extracted fermented product significantly reduced chicken peripheral blood mononuclear cells death induced by lipopolysaccharide and 2,2′-Azobis(2-amidinopropane) dihydrochloride. Birds received 5% and 10% FAC had higher weight gain than WB groups. Cecal coliform and lactic acid bacteria were diminished and increased respectively while diet replaced with FAC. For FAC supplemented groups, superoxide dismutase (SOD) activity increased at 35 days only, with catalase elevated at 21 and 35 day. Regarding serum lipid parameters, 10% FAC replacement significantly reduced triglyceride and low-density lipoprotein level in chickens. For fatty acid composition in pectoral superficial muscle of 35-d-old chickens, 5% and 10% FAC inclusion had birds with significantly lower saturated fatty acids as compared with 10% WB group. Birds on the 5% FAC diet had a higher degree of unsaturation, followed by 10% FAC, control, 5% WB, and 10% WB.
In conclusion, desirable intestinal microflora in chickens obtaining FAC may be attributed to the functional metabolites detected in final fermented product. Moreover, antioxidant effects observed in FAC were plausibly exerted in terms of improved antioxidant enzymes activities, increased unsaturated degree of fatty acids in chicken muscle and better weight gain in FAC inclusion groups, indicating that FAC possesses promising favorable mechanisms worthy to be developed.
論文出處：Int J Biol Macromol. 2018 Dec;120(Pt B):1551-1560. doi: 10.1016/j.ijbiomac.2018.09.162. Epub 2018 Sep 26.
論文名稱：A polysaccharide from Antrodia cinnamomea mycelia exerts antitumor activity through blocking of TOP1/TDP1-mediated DNA repair pathway.
作 者：Zhang Y, Wang Z, Li D, Zang W, Zhu H, Wu P, Mei Y, Liang Y.
出 版 年：2018
論文結論：A polysaccharide (termed ACPS-1) from mycelia of Antrodia cinnamomea under submerged culture was purified by hot water extraction and successive DEAE-52 cellulose and Sephadex G-100 column chromatography. ACPS-1 induced cancer cell lines (HeLa, A431, H22 and S180) apoptosis and cell cycle arrest (cells remained in G2/M phase) through blocking of topoisomerase I/tyrosyl-DNA phosphodiesterase I (TOP1/TDP1)-mediated DNA repair pathway.
出 版 年：2017
論文結論：風乾非萃取式小麥培養基固態發酵牛樟芝(WFAC)可以改善因四氯化碳引起大鼠慢性肝中毒病症，是四氯化碳慢性誘導肝損傷的無毒保肝劑。大鼠血漿中的天冬氨酸氨基轉移酶(aspartate aminotransferase)離子體和谷丙轉氨酶(alanine aminotransferase)水平的上升、同時顯著減少四氯化碳誘導增加肝臟脂質過氧化水平和羥脯氨酸(hydroxyproline)含量、並降低了脾臟重量和肝臟的水含量、也恢復了四氯化碳中毒大鼠肝臟可溶性蛋白質合成和血漿白蛋白的濃度，但絲毫不影響超氧化物歧化酶(superoxide dismutase)、過氧化氫酶，或穀胱甘肽過氧化物酶(glutathione peroxidase)的活動。
論文出處：Front Microbiol. 2020 Jul 3;11:1113. doi: 10.3389/fmicb.2020.01113.
論文名稱：Triterpenoids Extracted From Antrodia cinnamomea Mycelia Attenuate Acute Alcohol-Induced Liver Injury in C57BL/6 Mice via Suppression Inflammatory Response.
作 者：Liu Y, Wang Z, Kong F, Teng L, Zheng X, Liu X, Wang D.
出 版 年：2020
論文結論：Excessive alcohol consumption causes liver injury–induced mortality. Here we systematically analyzed the structure of triterpenoids extracted from Antrodia cinnamomea mycelia (ACT) and investigated their protective effects against acute alcohol-induced liver injury in mice. Liquid chromatography–mass spectrometry and liquid chromatography with tandem mass spectrometry were performed to determine the structures of ACT constituents. Alcohol-induced liver injury was generated in C57BL/6 mice by oral gavage of 13 g/kg white spirit (a wine at 56% ABV). Mice were treated with either silibinin or ACT for 2 weeks. Liver injury markers and pathological signaling were then quantified with enzyme-linked immunosorbent assays, antibody array assays, and Western blots, and pathological examinations were performed using hematoxylin-eosin staining and periodic acid–Schiff staining. Triterpenoids extracted from A. cinnamomea mycelia contain 25 types of triterpenoid compounds. A 2-weeks alcohol consumption treatment caused significant weight loss, liver dyslipidemia, and elevation of alanine aminotransferase, aspartate aminotransferase, γ-glutamyl transferase, and alkaline phosphatase activities in the serum and/or liver. These effects were markedly reversed after 2-weeks ACT administration. Triterpenoids extracted from A. cinnamomea mycelia alleviated the organ structural changes and inflammatory infiltration of alcohol-damaged tissues. Triterpenoids extracted from A. cinnamomea mycelia inhibited proinflammatory cytokine levels and enhanced anti-inflammatory cytokine levels. Acute alcohol treatment promoted inflammation with significant correlations to hypoxia-inducible factor 1α (HIF-1α), which was reduced by ACT and was partially related to modulation of the protein kinase B (Akt)/70-kDa ribosomal protein S6 kinase phosphorylation (p70S6K) and Wnt/β-catenin signaling pathways. In conclusion, ACT protected against acute alcohol-induced liver damage in mice mainly through its suppression of the inflammatory response, which may be related to HIF-1α signaling.
論文出處：Int J Mol Sci. 2018 Oct 10;19(10). pii: E3096. doi: 10.3390/ijms19103096.
論文名稱：Hypermethylation of CCND2 in Lung and Breast Cancer Is a Potential Biomarker and Drug Target.
作 者：Hung CS, Wang SC, Yen YT, Lee TH, Wen WC, Lin RK.
出 版 年：2018
出 版 年：2017
論文出處：Int J Nanomedicine. 2020 Jun 15;15:4191-4203. doi: 10.2147/IJN.S252885.
論文名稱：Nanoparticles of Antroquinonol-Rich Extract from Solid-State-Cultured Antrodia cinnamomea Improve Reproductive Function in Diabetic Male Rats.
作 者：Kong ZL, He JL, Sudirman S, Kuo MT, Miao S, Chang KB, Tsou D.
出 版 年：2020
論文結論：Purpose: To characterize the nanoparticle of antroquinonol from A. cinnamomea and its ameliorative effects on the reproductive dysfunction in the diabetic male rat.
Material and Methods: The chitosan-silicate nanoparticle was used as the carrier for the delivery of antroquinonol from solid-state-cultured A. cinnamomea extract (AC). The rats were fed with a high-fat diet and intraperitoneally injected with streptozotocin to induce diabetes. The rats were daily oral gavage by water [Diabetes (DM) and Control groups], three different doses of chitosan-silicate nanoparticle of antroquinonol from solid-state-cultured A. cinnamomea (nano-SAC, NAC): (DM+NAC1x, 4 mg/kg of body weight; DM+NAC2x, 8 mg/kg; and DM+NAC5x, 20 mg/kg), solid-state-cultured AC (DM+AC5x, 20 mg/kg), or metformin (DM+Met, 200 mg/kg) for 7 weeks.
Results: The nano-SAC size was 37.68± 5.91 nm, the zeta potential was 4.13± 0.49 mV, encapsulation efficiency was 79.29± 0.77%, and loading capacity was 32.45± 0.02%. The nano-SAC can improve diabetes-induced reproductive dysfunction by regulating glucose, insulin, and oxidative enzyme and by increasing the level of testosterone, follicle-stimulating hormone, luteinizing hormone, and sperm count as well as sperm mobility. In testicular histopathology, the seminiferous tubules of A. cinnamomea-supplemented diabetic rats showed similar morphology with the control group.
Conclusion: The nanoparticle of antroquinonol from Antrodia cinnamomea can be used as an effective strategy to improve diabetes-induced testicular dysfunction.
論文出處：Food Chem Toxicol. 2018 Feb 21. pii: S0278-6915(18)30028-0. doi: 10.1016/j.fct.2018.01.028. [Epub ahead of print]
論文名稱：Antrodia cinnamomea mycelial fermentation broth inhibits the epithelial-mesenchymal transition of human esophageal adenocarcinoma cancer cells.
作 者：Liu YM, Liu YK, Huang PI, Tsai TH, Chen YJ
出 版 年：2018
論文結論：Antrodia cinnamomea mycelial fermentation broth (AC-MFB) was not only able to upregulate the expression of Ecadherin and attenuate the TGF-β-induced overexpression of vimentin and N-cadherin, but it also reversed the TGF-β-induced changes in cell morphology from polygonal to spindle-shaped and delayed the migration potential of human esophageal cancer cells (BE3). And AC-MFB was able to inhibit the epithelial mesenchymal transition (EMT) of esophageal cancer BE3 cells, which was accompanied by Twist and Twist1 downregulation.
出 版 年：2017
論文結論：牛樟芝的新抗發炎化合物antrolone及antcamphin M能夠劑量依賴性地顯著減少因LPS所誘導的一氧化氮、前列腺素 (PGE2)、腫瘤壞死因子 (TNF-α)和第一介白素(IL-1β)的產生，以及誘導型NO合成酶 (iNOS)和環氧合酶-2 (COX-2)的蛋白濃度，且並未產生顯著細胞毒性。同時亦顯著抑制了MAPK和NF-κB路徑的活化，包括了p38MAPK、ERK和JNK、IκB-α以及核質分離後之p65等之蛋白濃度。並且使HO-1及Nrf2的表現量增加。
論文出處：Biomed Pharmacother. 2019 Apr;112:108684. doi: 10.1016/j.biopha.2019.108684. Epub 2019 Feb 21.
論文名稱：Attenuation of reproductive dysfunction in diabetic male rats with timber cultured Antrodia cinnamomea ethanol extract.
作 者：Johnson A, Cheng SC, Tsou D, Kong ZL.
出 版 年：2019
論文結論：Diabetes mellitus together with the oxidative stress affects the process of spermatogenesis and leads to male infertility. Antrodia cinnamomea ethanol extract (ACEE) has anti-inflammatory and ameliorative effects to prevent diabetes-induced male reproductive dysfunction. ACEE improved STZ-NA (streptozotocin (STZ) (65 mg/kg) and nicotinamide (NA) (230 mg/kg)) induced hyperglycemia, oxidative stress, and insulin resistance. ACEE reduced the degree of lipid peroxidation, recovered the abnormal structure of the seminiferous tubules, and improved sperm parameters.
論文出處：BMC Complement Altern Med. 2018 May 9;18(1):152. doi: 10.1186/s12906-018-2204-y.
論文名稱：Antrodia cinnamomea extract inhibits the proliferation of tamoxifen-resistant breast cancer cells through apoptosis and skp2/microRNAs pathway.
作 者：Lin YS, Lin YY, Yang YH, Lin CL, Kuan FC, Lu CN, Chang GH, Tsai MS, Hsu CM, Yeh RA, Yang PR, Lee IY, Shu LH, Cheng YC, Liu HT, Lee KD, Chang DC, Wu CY
出 版 年：2018
論文結論：The ethanol extract of antrodia cinnamomea can inhibit the growth of breast cancer cells and induce apoptosis in these breast cancer cells, including MCF-7 cell and tamoxifen-resistant MCF-7 cell lines. It could be a novel anticancer agent in the armamentarium of tamoxifen-resistant breast cancer management.
出 版 年：2017
論文出處：Int J Mol Sci. 2019 Feb 15;20(4). pii: E846. doi: 10.3390/ijms20040846.
論文名稱：Protective Effect of Antrodia cinnamomea Extract against Irradiation-Induced Acute Hepatitis.
作 者：Kuo TH, Kuo YH, Cho CY, Yao CJ, Lai GM, Chuang SE.
出 版 年：2019
論文結論：Radiotherapy for treatment of hepatocellular carcinoma causes severe side effects, including acute hepatitis and chronic fibrosis. In normal liver cell line CL48, ethanol extract of Antrodia cinnamomea (ACE) protects cells by eliminating irradiation-induced reactive oxygen species (ROS) through the induction of Nrf2 and the downstream redox system enzymes. The protective effect of ACE was also demonstrated in tumor-bearing mice by alleviating irradiation-induced acute hepatitis. ACE could also protect mice from CCl₄-induced hepatitis. ACE likely contains active components that protect normal liver cells from free radical attack and can potentially benefit hepatocellular carcinoma (HCC) patients during radiotherapy.
論文出處：Int J Mol Sci. 2018 May 24;19(6). pii: E1565. doi: 10.3390/ijms19061565.
論文名稱：Anti-Metastatic Effects of Antrodan with and without Cisplatin on Lewis Lung Carcinomas in a Mouse Xenograft Model.
作 者：Chen PC, Chen CC, Ker YB, Chang CH, Chyau CC, Hu ML
出 版 年：2018
論文結論：Antrodan, derived from the fungal mycelia of Antrodia cinnamomea, provides a novel, complementary therapeutic strategy against cancer metastasis, by attenuating the activities of MMP-2 and -9 through the modulation of STAT3/MAPK/ERK/JNK signaling pathways.
出 版 年：2017
論文結論：以油膜包覆之新劑型牛樟芝萃取物(NAE, New Antrodia cinnamomea )明顯降低大鼠血清中的促發炎因子：NO、ROS、、AST、ALT、IL-1 與 IL-6 含量與肝臟組織中誘導、第二型環氧化酶 (COX-2) 的蛋白質表現量。推斷NAE藉由抑制SD大鼠的肝臟 p-ERK、Caspase-3 與COX-2 表現量，進而降低肝臟發炎的程度，達到保護肝臟的功能。NAE 於四氯化碳誘導逆境實驗證實，對 SD 大鼠抗氧化與解毒代謝能力增強，並可降低肝臟氧化及發炎程度。